Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An assay for intercellular adhesive specificity.

S Roth, E J McGuire, S Roseman

    The Journal of Cell Biology
    |November 1, 1971
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Intercellular adhesion : I. A quantitative assay for measuring the rate of adhesion.

    The Journal of membrane biology·2013
    Same author

    Intercellular adhesion : II. The purification and properties of a horse serum protein that promotes neural retina cell aggregation.

    The Journal of membrane biology·2013
    Same author

    Implementation of the INTERGROWTH-21st Project in the UK.

    BJOG : an international journal of obstetrics and gynaecology·2013
    Same author

    Soft x-ray gain in the alkali earths.

    Applied optics·2010
    Same author

    Intercellular adhesion.

    Upsala journal of medical sciences·2010
    Same author

    The transport of carbohydrates by a bacterial phosphotransferase system.

    The Journal of general physiology·2009
    Same journal

    A pan-vertebrate signaling motif controls the molecular function of intracellular AQP12.

    The Journal of cell biology·2026
    Same journal

    Synergistic assembly, disassembly, and protection of complex forms of bundled F-actin.

    The Journal of cell biology·2026
    Same journal

    Recruitment and release of XPG during NER is controlled by pre- and post-incision factors and EXO1.

    The Journal of cell biology·2026
    Same journal

    Meiotic CENP-C supports centromere assembly and kinetochore recruitment in spermatogenesis.

    The Journal of cell biology·2026
    Same journal

    Phosphatidylserine and RhoB connect PI4P and PA metabolism to maintain plasma membrane identity.

    The Journal of cell biology·2026
    Same journal

    PIKfyve influences inter-organelle contacts with lysosomes to modulate the endoplasmic reticulum.

    The Journal of cell biology·2026
    See all related articles

    This study refines an assay for cell adhesion specificity using radioactive labeling. The modified method enhances speed and confirms that same-tissue cell collection (isotypic) exceeds different-tissue collection (heterotypic).

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Developmental Biology

    Background:

    • Understanding intercellular adhesive specificity is crucial for studying tissue development and function.
    • Existing assays for cell adhesion can be time-consuming and lack efficiency.

    Purpose of the Study:

    • To describe a modified, more rapid assay for quantifying intercellular adhesive specificity.
    • To investigate factors influencing cell collection by tissue aggregates and identify surface molecules involved in adhesion.

    Main Methods:

    • Radioactive labeling (using 32P) of tissue cells for enhanced detection.
    • Collection of labeled cells by isotypic and heterotypic tissue aggregates.
    • Liquid scintillation counting to quantify cell collection.
    • Enzymatic treatment of aggregates (proteases, glycosidases) to probe surface interactions.

    Related Experiment Videos

    Main Results:

    • The modified assay using 32P significantly accelerated the estimation of cell collection.
    • Isotypic cell collection was consistently higher than heterotypic collection across various chick embryo tissues.
    • Beta-galactosidase treatment of neural retina aggregates increased non-specific cell collection, suggesting its role in adhesion.

    Conclusions:

    • The refined assay provides a faster and more efficient method for studying cell adhesion.
    • Cell adhesion is specific, with a preference for homotypic interactions.
    • Beta-galactosidase may play a role in mediating specific cell-surface interactions in neural retina.