Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Flow cytometry: a high-resolution instrument for everyone.

H B Steen, T Lindmo

    Science (New York, N.Y.)
    |April 27, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A Case of Faciolopsis Buski: (Distoma Buski <i>v.</i> Crassum) and Amphistoma Hominis.

    The Indian medical gazette·2017
    Same author

    Large Renal Calculus.

    The Indian medical gazette·2017
    Same author

    Case of Molluscum Fibrosum with Definite Family History.

    The Indian medical gazette·2017
    Same author

    Monte Carlo study of in-field and out-of-field dose distributions from a linear accelerator operating with and without a flattening-filter.

    Medical physics·2012
    Same author

    Contralateral breast doses measured by film dosimetry: tangential techniques and an optimized IMRT technique.

    Physics in medicine and biology·2009
    Same author

    Flow cytometers for characterization of microorganisms.

    Current protocols in cytometry·2008
    Same journal

    Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

    Science (New York, N.Y.)·2026
    Same journal

    Local signals, systemic decline.

    Science (New York, N.Y.)·2026
    Same journal

    The mechanics of liver regeneration.

    Science (New York, N.Y.)·2026
    Same journal

    Computing in a memory with physics.

    Science (New York, N.Y.)·2026
    Same journal

    Retraction.

    Science (New York, N.Y.)·2026
    Same journal

    Making time.

    Science (New York, N.Y.)·2026
    See all related articles

    A novel flow cytometry configuration creates a stable, flat cell stream for precise DNA analysis. This easy-to-operate instrument achieves high resolution, enhancing cellular measurements.

    Area of Science:

    • Biotechnology
    • Cell Biology
    • Analytical Chemistry

    Background:

    • Flow cytometry is a crucial technique for analyzing cell characteristics.
    • Existing flow cytometer designs can present challenges in stability and ease of operation.
    • Accurate measurement of cellular DNA content is vital for various biological studies.

    Purpose of the Study:

    • To develop a new, stable, and user-friendly flow configuration for flow cytometry.
    • To improve the resolution and accuracy of cellular DNA measurements.
    • To present an innovative approach for analyzing stained cells.

    Main Methods:

    • A novel flow configuration was designed using a pressurized water jet to create a flat, laminar cell stream on a coverslip.
    • Stained cells were contained within a narrow sector of the water stream.

    Related Experiment Videos

  • Fluorescence microscopy with incident illumination and a pulse photometer, coupled to a multichannel pulse height analyzer, was employed for cell analysis.

    • Main Results:

    • The developed instrument demonstrated stable operation.
    • The flow cytometer achieved a resolution equal to or better than a coefficient of variance of 1.4% in cellular DNA measurements.
    • The system proved to be easy to operate.

    Conclusions:

    • The new flow configuration offers a stable and highly resolvable method for flow cytometry.
    • This technique provides an effective and accessible tool for accurate cellular DNA analysis.
    • The design represents a significant advancement in flow cytometry instrumentation.