Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mitochondrial DNA replication in sea urchin oocytes.

L Matsumoto, H Kasamatsu, L Pikó

    The Journal of Cell Biology
    |October 1, 1974
    PubMed
    Summary

    Sea urchin oocyte mitochondrial DNA (mtDNA) replication utilizes a displacement loop model with early, multiple initiations. This unique process, observed in non-dividing cells, results in abundant Cairns

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Expression of telomerase catalytic subunit (hTERT) mRNA does not predict survival in patients with transitional cell carcinoma of the upper urinary tract.

    Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc·2001
    Same author

    MDR1 bicistronic vectors: analysis of selection stringency, amplified gene expression, and vector stability in cell lines.

    Biochemical pharmacology·2001
    Same author

    Simian virus 40 Vp1 DNA-binding domain is functionally separable from the overlapping nuclear localization signal and is required for effective virion formation and full viability.

    Journal of virology·2001
    Same author

    Expression of p27(Kip1) protein in transitional cell carcinoma of the upper urinary tract.

    Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc·2001
    Same author

    Laparoscopic-assisted surgery for benign ovarian cyst in a young girl.

    The Journal of the American Association of Gynecologic Laparoscopists·2001
    Same author

    Factors affecting the volume of umbilical cord blood collections.

    Acta obstetricia et gynecologica Scandinavica·2001

    Area of Science:

    • Mitochondrial genetics
    • Molecular biology
    • Developmental biology

    Background:

    • Mitochondrial DNA (mtDNA) replication mechanisms vary across species.
    • Understanding mtDNA replication in oocytes is crucial for developmental processes.
    • Previous models, like those in mouse L cells, provide a basis for comparison.

    Purpose of the Study:

    • To investigate the characteristics of mitochondrial DNA (mtDNA) replication intermediates in Strongylocentrotus purpuratus oocytes.
    • To compare mtDNA replication in oocytes with established models, such as that in mouse L cells.
    • To identify novel structural variants of mtDNA during replication.

    Main Methods:

    • Isolation of mtDNA replicative intermediates using ethidium bromide-CsCl density gradient centrifugation.
    • Electron microscopy examination of mtDNA after formamide spreading.
    • Radioactive labeling of mtDNA using [(3)H]thymidine in isolated oocytes.

    Main Results:

    • Oocyte mtDNA replication follows a displacement loop (D-loop) model, but with a lower frequency of D-loop DNA, indicating shorter holding times.
    • Duplex synthesis on the displaced strand initiates early and occurs multiple times.
    • A high frequency of Cairns' forms (totally duplex replicative forms) was observed, the highest reported for mtDNA.
    • Expanded D loops suggest replication on a nicking-closing cycle-regulated circular template.
    • Mature sea urchin eggs contain predominantly circular duplex mtDNA with absent replicative forms.
    • Novel structural variants, including denaturation loops and double branch-migrated replicative forms, were identified.

    Conclusions:

    • Sea urchin oocyte mtDNA replication is characterized by early, multiple initiations and a high abundance of Cairns' forms, differing from mouse L cell models.
    • These differences may stem from replication occurring independently of the cell cycle in non-dividing oocytes.
    • Mature eggs store mtDNA as stable circular duplexes, with replication intermediates being transient.
    • The study reveals unique aspects of mtDNA replication and structural variations in sea urchin oocytes.

    Related Experiment Videos