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Red cell membrane permeability deduced from bulk diffusion coefficients.

W R Redwood, E Rall, W Perl

    The Journal of General Physiology
    |December 1, 1974
    PubMed
    Summary
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    This study determined red blood cell membrane permeability to water and amides using bulk diffusion. The novel method accurately measures permeability coefficients for rapidly permeating solutes.

    Area of Science:

    • Biophysics
    • Cell Biology
    • Physiology

    Background:

    • Understanding red blood cell membrane permeability is crucial for various physiological processes.
    • Previous methods for measuring membrane permeability can be complex or unsuitable for certain solutes.

    Purpose of the Study:

    • To develop and validate a simple method for determining red blood cell membrane permeability coefficients.
    • To measure the permeability of dog red blood cell membranes to tritiated water and various carbon-14 labeled amides.

    Main Methods:

    • Bulk diffusion measurements were performed on packed dog red blood cells within polyethylene tubing.
    • Radioactive tracers (tritiated water, C-14 amides) were used to monitor solute diffusion.
    • A one-dimensional diffusion model was applied, accounting for intracellular and extracellular pathways.

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    Main Results:

    • Permeability coefficients for tritiated water and several amides were successfully deduced.
    • The derived permeability coefficients showed satisfactory agreement with existing data obtained via rapid-reaction techniques.
    • The method proved effective, particularly for solutes with high membrane permeability.

    Conclusions:

    • The bulk diffusion method provides a simple and reliable approach to measure red blood cell membrane permeability.
    • This technique is well-suited for characterizing the permeability of rapidly permeating substances.
    • The findings contribute to a better understanding of solute transport across the red blood cell membrane.