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Related Experiment Videos

Solute-excluded volumes near the Novikoff cell surface.

T G Polefka, R A Garrick, W R Redwood

    The American Journal of Physiology
    |November 1, 1984
    PubMed
    Summary

    Novikoff hepatoma cells exhibit unique pericellular volumes that restrict solute access based on molecular weight. This size-exclusion phenomenon, driven by cell surface structures, differs from erythrocytes and fat cells.

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    Area of Science:

    • Cell Biology
    • Biophysics
    • Biochemistry

    Background:

    • The extracellular space surrounding cells plays a critical role in regulating molecular transport.
    • Understanding the pericellular domain is essential for comprehending cell-environment interactions.
    • Novikoff hepatoma cells possess distinct surface characteristics that may influence solute accessibility.

    Purpose of the Study:

    • To investigate the pericellular volume of Novikoff hepatoma cells using differential centrifugation.
    • To determine the relationship between molecular size and solute accessibility within the pericellular domain.
    • To compare the solute exclusion properties of Novikoff hepatoma cells with other cell types.

    Main Methods:

    • Differential centrifugation was employed to isolate extracellular water intimately associated with Novikoff hepatoma cells.

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  • The distribution volumes of various solutes (albumin, inulin, raffinose, sucrose) were measured.
  • Enzymatic treatments were used to selectively remove cell surface components.

    • Main Results:

    • Pericellular volumes accessible to solutes were inversely related to their molecular weights in Novikoff hepatoma cells.
    • This size-dependent exclusion was not observed in erythrocytes or fat cells.
    • Enzymatic removal of surface components altered solute distribution volumes proportionally.

    Conclusions:

    • Novikoff hepatoma cells exhibit size-selective exclusion of solutes within their pericellular domain.
    • The cell surface coat and microvilli are key contributors to this size-discriminating barrier.
    • These surface structures provide a unique level of molecular discrimination not found in all cell types.