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Updated: Jul 28, 2026

Functional Complementation Analysis (FCA): A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
09:27

Functional Complementation Analysis (FCA): A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways

Published on: June 24, 2016

Studies on plasmid replication. IV. Complementation of replication-defective mutants by an incompatibility-deficient

L Wyman, R P Novick

    Molecular & General Genetics : MGG
    |January 1, 1974
    PubMed

    This paper described a complementation test system for replication-defective S. aureus pencillinase plasmids in which the incompatibility barrier has been overcome by the isolation of an incompatibility-defective (Inc-) plasmid. This plasmid appears to be stably and irreversibly integrated into the host chromosome as attempts to restore it to its original independent state have been unsuccessful. The Inc- plasmid was able to complement the thermosensitive replication defects of Seg- plasmids belonging to the same original incompatibility class but was unable to complement onels belonging to a different incompatibility class. Positive and negative phenotypic complementation tests were confirmed at the molecular level by isotopec labeling of plasmid-specific DNA molecules.

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