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Lipogenesis in rabbit isolated fat-cells.

E D Saggerson

    The Biochemical Journal
    |September 1, 1974
    PubMed
    Summary
    This summary is machine-generated.

    Rabbit fat cells efficiently synthesize fatty acids from various substrates like glucose, pyruvate, and lactate. Their metabolic pathways suggest the citrate-cleavage pathway isn't essential for generating fatty acid building blocks.

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Metabolic Research

    Background:

    • Fatty acid synthesis is crucial for energy storage in adipose tissue.
    • Understanding substrate utilization in adipocytes provides insights into metabolic regulation.
    • Rabbit fat cells serve as a model for studying lipogenesis.

    Purpose of the Study:

    • To investigate substrate utilization and fatty acid synthesis in rabbit fat cells.
    • To determine the metabolic pathways involved in lipogenesis in rabbits.
    • To compare rabbit fat cell metabolism with that of rats and guinea pigs.

    Main Methods:

    • Incubation of isolated rabbit perirenal fat cells with U-(14)C-labelled substrates (glucose, pyruvate, lactate, acetate).
    • Measurement of substrate incorporation into glyceride fatty acids and CO2.

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  • Enzyme activity assays for key metabolic enzymes within extramitochondrial and mitochondrial compartments.

    • Main Results:

    • Rabbit fat cells demonstrated similar total fatty acid synthesis rates regardless of the substrate provided.
    • [U-(14)C]acetate was incorporated into fatty acids and CO2 in both the presence and absence of glucose.
    • Enzyme analysis revealed low pyruvate carboxylase and undetectable NADP-malate dehydrogenase activity, alongside high mitochondrial phosphoenolpyruvate carboxylase activity.
    • Rabbit fat cell metabolic parameters fell between those of rat and guinea pig fat cells.
    • Evidence suggests the citrate-cleavage pathway may not be obligatory for supplying lipogenic acetyl units in rabbit fat cells.

    Conclusions:

    • Rabbit fat cells exhibit versatile substrate utilization for fatty acid synthesis.
    • The metabolic enzyme profile in rabbit fat cells differs from other species, influencing lipogenic pathways.
    • Lipogenesis in rabbit fat cells may not strictly rely on the citrate-cleavage pathway for acetyl-CoA supply.