Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Messenger selection by bacterial ribosomes.

S Leffler, W Szer

    Proceedings of the National Academy of Sciences of the United States of America
    |August 1, 1973
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Modification of polyuridylic acid by bisulfite. II: Studies on ribosomal binding and enzymatic hydrolysis.

    Molecular biology reports·2013
    Same author

    Analysis of parental estimates of children's weights in the ED.

    Annals of emergency medicine·1997
    Same author

    Identification of N(G)-methylarginine residues in human heterogeneous RNP protein A1: Phe/Gly-Gly-Gly-Arg-Gly-Gly-Gly/Phe is a preferred recognition motif.

    Biochemistry·1997
    Same author

    Antibodies to the 45 kDa DEK nuclear antigen in pauciarticular onset juvenile rheumatoid arthritis and iridocyclitis: selective association with MHC gene.

    The Journal of rheumatology·1997
    Same author

    Comparison of bupivacaine and lidocaine/bupivacaine for local anesthesia/digital nerve block.

    Annals of emergency medicine·1996
    Same author

    A novel autoantibody to the putative oncoprotein DEK in pauciarticular onset juvenile rheumatoid arthritis.

    The Journal of rheumatology·1994
    Same journal

    Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Tomographic imaging of superconducting order using particle-hole interference.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Analytical modeling for suction cup designs for skin-interfaced wearable devices.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Improving cell-free metabolism through direct integration of artificial respiratory chains.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    See all related articles

    Researchers isolated Caulobacter crescentus initiation factor 3 (C-IF-3) and found it functionally substitutes for Escherichia coli IF-3 in translation. This suggests conserved roles for IF-3 in bacterial protein synthesis across species.

    Area of Science:

    • Molecular Biology
    • Bacteriology
    • Virology

    Background:

    • Initiation factor 3 (IF-3) is crucial for bacterial translation initiation.
    • Escherichia coli IF-3 plays a key role in distinguishing mRNA and binding ribosomal subunits.
    • Caulobacter crescentus is a model organism for studying bacterial cell cycle and development.

    Purpose of the Study:

    • To isolate and characterize the Caulobacter crescentus homolog of E. coli IF-3 (C-IF-3).
    • To investigate the functional role of C-IF-3 in in vitro translation.
    • To compare the translational activity of C-IF-3 and E. coli IF-3 using phage RNA.

    Main Methods:

    • Isolation and purification of C-IF-3 from Caulobacter crescentus.
    • In vitro translation assays using RNA from C. crescentus RNA phage Cb5 and coliphage MS2.

    Related Experiment Videos

  • Comparative analysis of C-IF-3 and E. coli IF-3 activity with homologous and heterologous ribosomes.

    • Main Results:

    • Purified C-IF-3 functionally substituted for E. coli IF-3 in promoting MS2 RNA translation by E. coli ribosomes.
    • E. coli IF-3 substituted for C-IF-3 in Cb5 RNA translation by C. crescentus ribosomes.
    • Both phage RNAs required host ribosomes or mixed ribosomes with host 30S subunits for translation.
    • C-IF-3 was shown to dissociate both C. crescentus and E. coli 70S ribosomes and bind various RNA types.

    Conclusions:

    • C-IF-3 is functionally conserved with E. coli IF-3, indicating a conserved role in translation initiation.
    • Ribosomal subunit compatibility is essential for phage RNA translation, highlighting species-specific interactions.
    • IF-3's ability to dissociate ribosomes and bind RNA is a conserved mechanism across bacterial species.