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Antigenic properties of murine sarcoma virus-transformed BALB-3T3 nonproducer cells
Abstract:
The isolation of clonal lines of murine sarcoma virus-transformed, non-producer BALB/3T3 cells has provided a model system for determining whether RNA tumor virus-transformed cells possess virus-specific transplantation antigens. MSV nonproducer cells (K-234) were clonally derived from an inbred mouse cell line, BALB/3T3. A parallel virus-producing cell line was obtained by infection of the MSV nonproducer cells with Rauscher leukemia virus. K-234 was much more tumorigenic than K-234(R). Preimmunization of syngeneic mice with either K-234(R) or with UV-inactivated Rauscher leukemia virus induced transplantation resistance to subsequent challenge with K-234(R), but not with K-234. In contrast, mice preimmunized with nonproducer cells were not made resistant to subsequent challenge with the homologous cells. Antisera prepared from mice immunized with K-234(R) were specifically cytotoxic and positive by fluorescent antibody staining for K-234(R) target cells, but not to either BALB/3T3 or K-234. The results show that MSV nonproducer cells lack detectable transplantation antigens and suggest that the transplantation resistance to the producing cells is attributable to maturing virus at the cell surface.
Insights
Murine sarcoma virus (MSV) nonproducer cells lack detectable transplantation antigens. Transplantation resistance in virus-producing cells is attributed to maturing virus on the cell surface, not inherent cell antigens.
Area of Science:
- Oncology
- Virology
- Immunology
Background:
- Murine sarcoma virus (MSV)-transformed cells are a model for studying tumor virus antigens.
- Non-producer cells (K-234) were derived from BALB/3T3 cells, and a virus-producing line (K-234(R)) was created by Rauscher leukemia virus infection.
Purpose of the Study:
- To determine if RNA tumor virus-transformed cells possess virus-specific transplantation antigens.
- To investigate the role of cell-surface viral antigens in tumor rejection.
Main Methods:
- Isolation of MSV nonproducer (K-234) and virus-producing (K-234(R)) BALB/3T3 cell lines.
- Tumorigenicity assays and preimmunization studies in syngeneic mice.
- Cytotoxicity assays and fluorescent antibody staining using antisera from immunized mice.
Main Results:
- K-234 cells were more tumorigenic than K-234(R) cells.
- Preimmunization with K-234(R) or UV-inactivated Rauscher leukemia virus induced resistance to K-234(R) challenge, but not K-234 challenge.
- Antisera against K-234(R) were cytotoxic to K-234(R) cells but not to BALB/3T3 or K-234 cells.
Conclusions:
- MSV nonproducer cells lack detectable transplantation antigens.
- Transplantation resistance to virus-producing cells is likely due to maturing virus expressed on the cell surface.