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Crossed immuno-electrodiffusion in the diagnosis of immunoglobulin fragment abnormalities
Abstract:
The use of crossed immuno-electrodiffusion in the diagnosis of alpha-chain disease is described. The same basic technique is applicable in diagnosis of diseases in which immunoglobulin fragment abnormalities may occur. Crossed immuno-electrodiffusion is shown to be a superior method for demonstrating the abnormaility by providing more powerful resolution. Immunoselection (an adaptation of the classical method of immuno-electrophoresis) or rocket immuno-electrodiffusion both have limitations which are overcome by the use of crossed immuno-electrodiffusion. Mixture of antibodies is avoided by separating antibody-containing gels with interposition of intermediate gel layers free of antibody. Furthermore, precipitin rockets of some specific proteins can also be identified morphologically.
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