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Plasmid-associated functions of a stable Flac.

F L Macrina, E Balbinder

    Journal of Bacteriology
    |January 1, 1973
    PubMed
    Summary

    The stable FlacS plasmid in Escherichia coli alters sex pili, affecting phage adsorption but maintaining other functions. Its stability is not due to high copy numbers, and it appears larger than its precursor.

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    Area of Science:

    • Molecular Biology
    • Microbiology
    • Genetics

    Background:

    • Plasmids play crucial roles in bacterial adaptation and evolution.
    • Understanding plasmid stability and function is vital in microbial genetics.
    • The FlacS plasmid's characteristics and functions require detailed investigation.

    Purpose of the Study:

    • To investigate the functions associated with the stable FlacS plasmid.
    • To characterize the impact of FlacS on Escherichia coli.
    • To elucidate the mechanisms underlying FlacS stability.

    Main Methods:

    • Analysis of sex pili synthesis and male-specific phage adsorption in E. coli strains carrying FlacS.
    • Assessment of FlacS-mediated entry exclusion and plasmid incompatibility.
    • Detection of covalently closed circular deoxyribonucleic acid (cccDNA) to confirm autonomous replication.
    • Quantification of beta-galactosidase levels and cccDNA percentage to evaluate plasmid stability.
    • Sedimentation analysis in alkaline sucrose gradients to determine plasmid size.

    Main Results:

    • Escherichia coli strains with FlacS exhibit altered sex pili, leading to inefficient adsorption of male-specific phages.
    • FlacS-mediated entry exclusion and plasmid incompatibility functions remain normal.
    • The presence of cccDNA confirms FlacS as an autonomously replicating plasmid.
    • Plasmid stability is not attributed to high copy numbers, as indicated by beta-galactosidase levels and cccDNA percentage.
    • FlacS is larger than its precursor, F(ts114)lac, based on sedimentation analysis.

    Conclusions:

    • The FlacS plasmid influences specific aspects of E. coli physiology, notably sex pilus structure and phage interactions.
    • FlacS maintains essential plasmid functions like replication, entry exclusion, and incompatibility.
    • Plasmid stability is likely governed by mechanisms other than high-copy-number replication.
    • FlacS represents a distinct genetic entity with unique structural and functional properties compared to its precursor.

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