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Auxotab--a device for identifying enteric bacteria.

D L Rhoden, K M Tomfohrde, P B Smith

    Applied Microbiology
    |February 1, 1973
    PubMed
    Summary
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    The Auxotab multitest system correctly identified 87% of Enterobacteriaceae strains in a study. Further modifications are needed to improve its ease of use and accuracy for identifying enteric bacteria.

    Area of Science:

    • Microbiology
    • Clinical Diagnostics

    Background:

    • Accurate identification of enteric bacteria is crucial for clinical diagnostics and public health.
    • Rapid and reliable multitest systems can aid in bacterial identification.
    • The Auxotab system was developed as a potential tool for Enterobacteriaceae identification.

    Purpose of the Study:

    • To evaluate the performance of the Auxotab multitest system for the identification of Enterobacteriaceae.
    • To assess the accuracy and reliability of the Auxotab system in a double-blind study setting.

    Main Methods:

    • A multitest system, Auxotab, utilizing ten dehydrated reagents on a paper card was employed.
    • The system was evaluated using 417 known stock cultures of Enterobacteriaceae.
    • Double-blind studies were conducted to assess identification accuracy.

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    Main Results:

    • The Auxotab system correctly identified 87% of the tested Enterobacteriaceae strains.
    • The study identified areas for improvement in the Auxotab system's performance.

    Conclusions:

    • While the Auxotab system shows promise, modifications are necessary to enhance its ease of handling and reduce the time required for use.
    • Improvements in the accuracy of enteric bacteria identification are recommended for the Auxotab system to be a more effective diagnostic tool.