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Plasmids and transposable elements in Salmonella wien.

F Maimone, B Colonna, P Bazzicalupo

    Journal of Bacteriology
    |August 1, 1979
    PubMed
    Summary

    Characterization of Salmonella wien plasmids revealed three types, including large R plasmids conferring resistance to multiple antibiotics. These findings support a clonal distribution of S. wien in Europe and Algeria.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Salmonella wien is a serotype with clinical significance.
    • Plasmids play a crucial role in bacterial adaptation and antibiotic resistance.
    • Understanding plasmid profiles aids in tracking bacterial dissemination.

    Purpose of the Study:

    • To characterize the plasmids present in clinical strains of Salmonella wien.
    • To investigate the genetic features and antibiotic resistance profiles of these plasmids.
    • To assess the implications for the clonal distribution of S. wien.

    Main Methods:

    • Plasmid DNA extraction and characterization from six clinical S. wien strains.
    • Analysis of plasmid size, type (e.g., IncFI), and antibiotic resistance markers (tetracycline, chloramphenicol, kanamycin, ampicillin, sulfonamide, streptomycin).

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  • Investigation of transposable elements (TnA) and beta-lactamase genes.

    • Main Results:

    • All S. wien strains harbored three plasmid types: a large IncFI R-Tc Cm Km Ap plasmid, a smaller R-Ap Su Sm plasmid, and a cryptic plasmid.
    • Conjugative R plasmids showed characteristics suggesting recombination with F'lac pro, indicating donor phenotype regions.
    • Transposable element analysis identified TnA elements on both R plasmids, carrying TEM-type beta-lactamase genes.

    Conclusions:

    • The molecular and genetic similarity of R plasmids suggests a clonal origin for S. wien strains in Algeria and Europe.
    • The presence of TnA elements and beta-lactamase genes highlights potential mechanisms for antibiotic resistance spread.
    • Plasmid profiling provides valuable insights into the epidemiology and genetic makeup of Salmonella wien.