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Related Experiment Videos

Tetramethylbenzidine--a substitute for benzidine in hemoglobin analysis.

R C Lijana, M C Williams

    The Journal of Laboratory and Clinical Medicine
    |August 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

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    A new method using 3,3

    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry

    Background:

    • Plasma hemoglobin measurement is crucial for diagnosing certain medical conditions.
    • The traditional benzidine method for plasma hemoglobin is effective but poses significant health risks due to its carcinogenicity.
    • There is a clinical need for a safer alternative to the benzidine assay.

    Purpose of the Study:

    • To develop and validate a novel, non-carcinogenic assay for plasma hemoglobin.
    • To establish a reliable method for quantifying hemoglobin in plasma within a clinically relevant range.
    • To compare the performance of the new assay against the established benzidine method.

    Main Methods:

    • A colorimetric assay was developed utilizing 3,3',5,5'-tetramethylbenzidine (TMB) as a substitute for benzidine.
    • Hemoglobin concentrations in plasma samples were determined using the TMB method within the range of 1 to 100 mg/dl.

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  • Absorption spectra were analyzed to assess interference from plasma components.

    • Main Results:

    • The TMB method provides accurate hemoglobin determinations in the 1-100 mg/dl range.
    • The TMB assay demonstrated significantly lower interference from plasma proteins compared to the benzidine method.
    • The sensitivity of the TMB method was found to be at least seven times greater than that of the benzidine method.

    Conclusions:

    • 3,3',5,5'-tetramethylbenzidine is a safe and effective substitute for benzidine in plasma hemoglobin assays.
    • The developed TMB method offers improved accuracy and sensitivity for clinical hemoglobin measurements.
    • This non-carcinogenic assay provides a viable alternative for routine clinical laboratory use.