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Related Experiment Videos

Colorimetric acetaminophen determination in pharmaceutical formulations.

S Belal, M A Elsayed, A El-Waliely

    Journal of Pharmaceutical Sciences
    |June 1, 1979
    PubMed
    Summary
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    This study presents a new colorimetric method for determining acetaminophen (a common pain reliever) using copper(II) chelation. The enhanced method offers improved accuracy and sensitivity for pharmaceutical analysis.

    Area of Science:

    • Analytical Chemistry
    • Pharmaceutical Analysis

    Background:

    • Accurate quantification of acetaminophen is crucial for pharmaceutical quality control.
    • Existing methods for acetaminophen determination may lack sufficient selectivity or sensitivity.

    Purpose of the Study:

    • To develop and optimize novel colorimetric methods for acetaminophen determination.
    • To enhance the selectivity and sensitivity of acetaminophen assays through copper(II) chelation.

    Main Methods:

    • Colorimetric determination of acetaminophen via coupling with diazotized o-nitroaniline.
    • Copper(II) chelation of the coupled product to form a stable chelate.
    • Indirect determination of acetaminophen by measuring the chelate's copper content.
    • Spectrophotometric analysis to establish rectilinear relationships between absorbance and acetaminophen concentration.

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    Main Results:

    • The developed methods demonstrate high selectivity for acetaminophen due to copper(II) chelation.
    • Indirect determination via copper content significantly enhances assay sensitivity.
    • Optimum conditions for the colorimetric reactions and chelation were established.
    • Rectilinear calibration curves were obtained, enabling accurate quantification in pharmaceutical formulations.
    • The proposed methods provide more accurate results compared to the official method.

    Conclusions:

    • The novel colorimetric approaches, particularly the indirect method involving copper(II) chelation, offer a sensitive and selective means for acetaminophen quantification.
    • These methods are suitable for the accurate determination of acetaminophen in various pharmaceutical products.
    • The findings suggest an improvement over existing official methods for acetaminophen analysis.