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Indirect ultraviolet-reactivation of phage lambda.

J George, R Devoret, M Radman

    Proceedings of the National Academy of Sciences of the United States of America
    |January 1, 1974
    PubMed
    Summary
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    Conjugation in Escherichia coli K12 significantly enhances DNA repair and mutagenesis of damaged phage lambda. This indirect ultraviolet-reactivation suggests an inducible, error-prone DNA repair mechanism in bacteria.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Escherichia coli K12 is a model organism for studying bacterial genetics and DNA repair mechanisms.
    • Ultraviolet (UV) irradiation induces DNA damage, necessitating efficient repair pathways.
    • Bacterial conjugation facilitates horizontal gene transfer and can influence recipient cell physiology.

    Purpose of the Study:

    • To investigate the phenomenon of indirect ultraviolet-reactivation in Escherichia coli K12.
    • To elucidate the role of conjugation in DNA repair and mutagenesis of UV-damaged phage lambda.
    • To provide evidence for an inducible, error-prone DNA repair mechanism.

    Main Methods:

    • Utilizing F(-) recipient and Hfr or F-lac(+) donor strains of Escherichia coli K12.

    Related Experiment Videos

  • Employing ultraviolet-irradiated donors and phage lambda.
  • Assessing the capacity for DNA repair and mutagenesis in recipient bacteria.

    • Main Results:

    • Conjugation with UV-irradiated donors substantially increased the recipient's capacity for DNA repair and mutagenesis of UV-damaged phage lambda.
    • The observed phenomenon, termed indirect ultraviolet-reactivation, functions in pyrimidine dimer excision-deficient strains.
    • This process produces clear plaque mutations in the restored phage and is promoted by conjugation.

    Conclusions:

    • Indirect ultraviolet-reactivation in E. coli K12 is a conjugation-promoted process.
    • This phenomenon provides evidence for an inducible, error-prone DNA repair mechanism in bacteria.
    • The findings contribute to understanding bacterial responses to DNA damage and horizontal gene transfer.