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Vitrectomy in experimental endophthalmitis: Part I-Fungal infection
Abstract:
In studying the dose-related ocular toxicity of amphotericin B in vitrectomized eyes, up to 5 micrograms/0.1 ml concentration of amphotericin C was nontoxic to the ocular structure when injected in the anterior part of the vitreous cavity. Intravitreal injection of 2.5 micrograms of amphotericin B halted fungal endophthalmitis, which was experimentally induced in rabbit eyes, even after 16 days of infection. Combined treatment of vitrectomy and intravitreal injection of amphotericin B not only cured the fungal endophthalmitis but it also cleared the ocular media from opacities.
Insights
Amphotericin B effectively treats fungal endophthalmitis in rabbit eyes. Combined with vitrectomy, it clears infections and improves ocular media clarity.
Area of Science:
- Ophthalmology
- Mycology
- Pharmacology
Background:
- Fungal endophthalmitis poses a significant threat to vision.
- Amphotericin B is a potent antifungal agent.
- Vitrectomy is a surgical procedure to remove vitreous humor.
Purpose of the Study:
- To evaluate the ocular toxicity of amphotericin B.
- To determine the efficacy of intravitreal amphotericin B in treating fungal endophthalmitis.
- To assess the combined effect of vitrectomy and amphotericin B.
Main Methods:
- Dose-related ocular toxicity study of amphotericin B in vitrectomized rabbit eyes.
- Intravitreal injection of amphotericin B to treat experimentally induced fungal endophthalmitis.
- Evaluation of combined vitrectomy and intravitreal amphotericin B treatment.
Main Results:
- Amphotericin B up to 5 micrograms/0.1 ml was non-toxic in vitrectomized eyes.
- A 2.5 microgram dose of amphotericin B halted fungal endophthalmitis for 16 days.
- Combined treatment successfully cured fungal endophthalmitis and cleared ocular media opacities.
Conclusions:
- Intravitreal amphotericin B is a safe and effective treatment for fungal endophthalmitis.
- Combining vitrectomy with intravitreal amphotericin B offers superior outcomes.
- This combined approach can restore visual clarity in affected eyes.