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Search for C-type particles in human neoplasia.
Annals of Clinical and Laboratory Science
|January 1, 1975
Summary
Researchers reviewed RNA tumor virus characteristics, finding C-type particles in human cancers. These particles, observed in fibrosarcoma tissue cultures, showed elevated reverse transcriptase activity and 70S RNA.
Area of Science:
- Oncology
- Virology
- Molecular Biology
Background:
- RNA tumor viruses, or oncornaviruses, possess distinct biologic and morphologic features.
- C-type particles, prevalent in animal species, have been detected in human leukemias and sarcomas.
Purpose of the Study:
- To review the characteristics of oncornaviruses.
- To investigate the presence and nature of C-type particles in human tumors, specifically an infantile fibrosarcoma.
Main Methods:
- Detailed ultrastructural illustration of replicating oncornaviruses.
- Survey of human sarcomas and leukemias for C-type particles.
- Culturing of tissue from an infantile fibrosarcoma.
- Analysis of viral particles, reverse transcriptase activity, and RNA in tissue cultures.
Main Results:
- C-type particles were identified in human sarcomas and leukemias.
- Tissue cultures from an infantile fibrosarcoma yielded viral particles.
- Elevated reverse transcriptase activity and 70S RNA were detected in these cultures.
- The observed viral particles measured 125–150nm, larger than murine or avian Type C particles.
Conclusions:
- The findings support the presence of oncornaviruses in human malignancies.
- The larger size of these C-type particles distinguishes them from known animal counterparts.