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Bacillus cereus-induced fluid accumulation in rabbit ileal loops
Applied Microbiology
|September 1, 1972
Summary
The ligated rabbit ileal loop model effectively identifies Bacillus cereus strains causing food poisoning by detecting fluid accumulation. Optimal growth conditions, like Brain Heart Infusion broth, enhance this model's utility.
Area of Science:
- Microbiology
- Gastroenterology
- Food Safety
Background:
- Bacillus cereus is a common cause of foodborne illness.
- Developing reliable experimental models is crucial for understanding food poisoning mechanisms.
Purpose of the Study:
- To evaluate the ligated rabbit ileal loop as a model for Bacillus cereus food poisoning.
- To identify factors influencing the model's responsiveness.
Main Methods:
- Inoculation of ligated rabbit ileal loops with various Bacillus strains.
- Assessment of fluid accumulation as a response indicator.
- Testing different growth media and culture conditions.
Main Results:
- 19 of 22 B. cereus strains induced fluid accumulation.
- Brain Heart Infusion broth (BHI) supported optimal loop-positive responses.
- Fluid-inducing activity was associated with bacterial cells and filtrates from exponentially growing cultures within a specific temperature range (18-43°C).
- Intraluminal injection of B. cereus did not cause diarrhea.
Conclusions:
- The ligated rabbit ileal loop is a valuable model for B. cereus food poisoning research.
- Growth medium and bacterial growth phase significantly impact model outcomes.
- The model detects enterotoxin production but not necessarily diarrheal effects.