Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spiral plate method for bacterial determination.

J E Gilchrist, J E Campbell, C B Donnelly

    Applied Microbiology
    |February 1, 1973
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Assessing coverage of the monitoring framework of the Kunming-Montreal Global Biodiversity Framework and opportunities to fill gaps.

    Nature ecology & evolution·2025
    Same author

    Green turtles shape the seascape through grazing patch formation around habitat features: Experimental evidence.

    Ecology·2022
    Same author

    GIP mediates the incretin effect and glucose tolerance by dual actions on α cells and β cells.

    Science advances·2021
    Same author

    Sustained release of a GLP-1 and FGF21 dual agonist from an injectable depot protects mice from obesity and hyperglycemia.

    Science advances·2020
    Same author

    Precision Parameters for AOAC Bacillus stearothermophilus Disc Assay Based on FDA Milk Laboratory Quality Assurance 1982-1986 Samples.

    Journal of food protection·2019
    Same author

    Revision of Section 2.4M in Standard Methods for the Examination of Dairy Products, 15th Edition <sup>1</sup>.

    Journal of food protection·2019

    The spiral plate method efficiently estimates bacterial counts in solutions, requiring less time and materials than traditional pour plate methods. This innovative technique offers comparable variance for bacterial quantification in water and milk samples.

    Area of Science:

    • Microbiology
    • Analytical Chemistry

    Background:

    • Accurate bacterial enumeration is crucial for quality control in various industries.
    • Conventional methods like the pour plate procedure can be time-consuming and resource-intensive.

    Purpose of the Study:

    • To introduce and evaluate a novel spiral plate method for bacterial quantification.
    • To compare the efficiency and accuracy of the spiral plate method against the conventional pour plate procedure.

    Main Methods:

    • A machine deposits a known sample volume onto an agar plate in an Archimedes spiral pattern.
    • Incubation reveals varying colony densities, analyzed using a modified counting grid relating plate area to sample volume.
    • The spiral plate method was validated using pure and mixed bacterial cultures in water and milk.

    Related Experiment Videos

    Main Results:

    • The spiral plate method demonstrated comparable duplicate variance to the pour plate method at the alpha = 0.01 level for bacterial concentrations ranging from 600 to 12 x 10(5) bacteria/ml.
    • Higher bacterial counts were generally observed with the spiral plate method compared to the pour plate method.
    • Significantly reduced time and material requirements were noted for the spiral plate method.

    Conclusions:

    • The spiral plate method provides an efficient and cost-effective alternative for bacterial enumeration.
    • This method is suitable for quantifying bacteria in diverse sample matrices like water and milk.
    • Further research may optimize counting strategies for enhanced accuracy.