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Effect of sodium polyanetholesulfonate on antimicrobial systems in blood
Abstract:
Sodium polyanetholesulfonate (SPS), an anticoagulant which inhibits the antimicrobial systems of blood, is used widely in blood culture media. The addition of SPS to experimental blood cultures inoculated with small numbers of a variety of organisms caused a striking increase in recovery of these organisms. Sodium fluoride also increased the incidence of positive blood cultures with some organisms. SPS completely inhibited serum antibacterial activity and serum-dependent phagocytosis (and killing) by isolated leukocytes at a concentration usually employed in blood culture media. SPS also stimulated both glucose C-1 oxidation in resting leukocytes and formate oxidation in both resting and phagocytosing leukocytes in serum-free systems. These in vitro studies support the concept that SPS is a useful additive to blood culture media and further elaborate on the mechanism of its inhibition of the microbicidal activity of blood.
Insights
Sodium polyanetholesulfonate (SPS) enhances the recovery of organisms in blood cultures by inhibiting blood
Area of Science:
- Microbiology
- Immunology
- Hematology
Background:
- Sodium polyanetholesulfonate (SPS) is a common anticoagulant in blood culture media.
- SPS is known to inhibit certain antimicrobial systems present in blood.
Purpose of the Study:
- To evaluate the efficacy of SPS in improving the recovery of microorganisms from blood cultures.
- To elucidate the mechanisms by which SPS affects the antimicrobial activity of blood and leukocyte function.
Main Methods:
- Experimental blood cultures were inoculated with various organisms in the presence of SPS.
- In vitro assays were performed to assess serum antibacterial activity, phagocytosis, and leukocyte oxidation.
- The effects of SPS on resting and phagocytosing leukocytes were examined in serum-free systems.
Main Results:
- SPS significantly increased the recovery of inoculated organisms in experimental blood cultures.
- SPS completely inhibited serum antibacterial activity and serum-dependent phagocytosis and killing by leukocytes.
- SPS stimulated glucose and formate oxidation in leukocytes under specific in vitro conditions.
Conclusions:
- SPS is a valuable additive for blood culture media, enhancing microbial detection.
- SPS exerts its beneficial effect by neutralizing blood's microbicidal activity and modulating leukocyte function.