Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Myeloperoxidase.

S J Klebanoff1

  • 1Department of Medicine, University of Washington, Seattle 98195-7185, USA.

Proceedings of the Association of American Physicians
|October 16, 1999
PubMed
Summary
This summary is machine-generated.

The myeloperoxidase (MPO) system, using hydrogen peroxide (H2O2), destroys microbes but can also damage host tissues. Defects in MPO or H2O2 production impair microbicidal activity and contribute to disease pathogenesis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stimulation of human neutrophils and monocytes by staphylococcal phenol-soluble modulin.

Journal of leukocyte biology·2001
Same author

Cutting edge: functional interactions between toll-like receptor (TLR) 2 and TLR1 or TLR6 in response to phenol-soluble modulin.

Journal of immunology (Baltimore, Md. : 1950)·2000
Same author

Vaginal myeloperoxidase and flora in the pig-tailed macaque.

Journal of medical primatology·2000
Same author

NFAT1 enhances HIV-1 gene expression in primary human CD4 T cells.

Clinical immunology (Orlando, Fla.)·2000
Same author

An inflammatory polypeptide complex from Staphylococcus epidermidis: isolation and characterization.

The Journal of experimental medicine·1999
Same author

Lactobacilli and vaginal host defense: activation of the human immunodeficiency virus type 1 long terminal repeat, cytokine production, and NF-kappaB.

The Journal of infectious diseases·1999
Same journal

Predictors and outcomes of cardiac complications following elective coronary bypass grafting.

Proceedings of the Association of American Physicians·1999
Same journal

Prospective measure of serum 3-nitrotyrosine levels in systemic lupus erythematosus: correlation with disease activity.

Proceedings of the Association of American Physicians·1999
Same journal

Physiology and pathophysiology of nitric oxide in chronic renal disease.

Proceedings of the Association of American Physicians·1999
Same journal

Human herpesvirus 8: is it a tumor virus?

Proceedings of the Association of American Physicians·1999
Same journal

Hepatitis viruses: their role in human cancer.

Proceedings of the Association of American Physicians·1999
Same journal

Papillomaviruses in human cancers.

Proceedings of the Association of American Physicians·1999
See all related articles

Area of Science:

  • Immunology
  • Biochemistry
  • Cell Biology

Background:

  • Phagocytes utilize a respiratory burst, producing superoxide anion and hydrogen peroxide (H2O2), to combat pathogens.
  • Myeloperoxidase (MPO), released from neutrophils and monocytes, utilizes H2O2 to generate potent oxidants.
  • These oxidants, including hypochlorous acid, are crucial for microbial destruction but can also harm host tissues.

Purpose of the Study:

  • To elucidate the role of the myeloperoxidase (MPO)-hydrogen peroxide (H2O2)-chloride system in phagocyte function.
  • To investigate the consequences of MPO and H2O2 production on both microbial killing and host tissue damage.
  • To understand the implications of MPO deficiency and chronic granulomatous disease (CGD) on immune response and disease.

Main Methods:

Related Experiment Videos

  • Analysis of phagocyte respiratory burst activity and H2O2 production.
  • Assessment of MPO release and its enzymatic activity.
  • Evaluation of microbicidal function in neutrophils from healthy individuals and patients with MPO deficiency or CGD.
  • Investigation of MPO system's role in potential tissue damage mechanisms.

    • Main Results:

    • The MPO-H2O2-chloride system effectively kills ingested microorganisms within phagocytes.
    • Neutrophils lacking MPO or the respiratory burst (seen in CGD) exhibit impaired microbicidal activity.
    • Extracellular release of MPO and H2O2 can lead to oxidative damage to adjacent host tissues.
    • The MPO system is implicated in the pathogenesis of conditions like pulmonary injury and atherosclerosis.

    Conclusions:

    • The MPO system is a critical component of the innate immune response for microbial killing.
    • Dysregulation of the MPO system, due to deficiency or excessive activity, contributes to both impaired immunity and tissue injury.
    • Understanding the dual role of MPO is essential for comprehending host defense and inflammatory diseases.