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Electrical fluctuations associated with active transport.

J R Segal

    Biophysical Journal
    |November 1, 1972
    PubMed
    Summary
    This summary is machine-generated.

    Voltage fluctuations during active sodium transport in frog skin arise from both resistance and current changes. Ouabain affects current but not resistance fluctuations, indicating distinct mechanisms.

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    Area of Science:

    • Physiology
    • Biophysics
    • Membrane Transport

    Background:

    • Active transport of sodium ions across biological membranes is crucial for cellular function.
    • Voltage fluctuations are inherent in biological systems and can provide insights into transport mechanisms.
    • Frog abdominal skin is a well-established model for studying epithelial sodium transport.

    Purpose of the Study:

    • To investigate the origins and characteristics of voltage fluctuations during active sodium transport in frog abdominal skin.
    • To differentiate the contributions of resistance and current fluctuations to the overall voltage fluctuations.
    • To elucidate the role of specific transport components and temperature on these fluctuations.

    Main Methods:

    • Spectral analysis of voltage fluctuations in the 0.025-10 Hz band.

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  • Manipulation of transmembrane potential using an external current supply.
  • Application of Ouabain to selectively inhibit Na+/K+-ATPase.
  • Measurements conducted at different temperatures (20°C and 32°C).

    • Main Results:

    • Voltage fluctuations diminished with decreasing potential but reached a minimum finite value, indicating contributions from both resistance and current.
    • Ouabain abolished current fluctuations but not resistance fluctuations.
    • At 20°C, spectral intensities of resistance and current fluctuations followed a power law (1/f^a, a=1.6-2.0).
    • At 32°C, a sigmoid-shaped spectrum suggested a relaxation process with a time constant of 0.6 seconds.

    Conclusions:

    • Voltage fluctuations during active sodium transport are a composite of stochastic variations in membrane resistance and transport current.
    • The findings suggest a carrier-mediated mechanism for sodium transport, involving stochastic variations in carrier-ligand complex concentrations.
    • Temperature influences the dynamics of these fluctuations, potentially reflecting changes in carrier kinetics or membrane properties.