Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Isolation and structural studies on synaptic complexes from rat brain.

C W Cotman, D Taylor

    The Journal of Cell Biology
    |December 1, 1972
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Physical activity elicits sustained activation of the cyclic AMP response element-binding protein and mitogen-activated protein kinase in the rat hippocampus.

    Neuroscience·2001
    Same author

    Oxidation of Abeta and plaque biogenesis in Alzheimer's disease and Down syndrome.

    Neurobiology of disease·2001
    Same author

    Estrogen regulates bcl-x expression in rat hippocampus.

    Neuroreport·2001
    Same author

    Bcl-2 family protein behavior in frontotemporal dementia implies vascular involvement.

    Neurology·2001
    Same author

    Activated caspase-3 expression in Alzheimer's and aged control brain: correlation with Alzheimer pathology.

    Brain research·2001
    Same author

    Complement association with neurons and beta-amyloid deposition in the brains of aged individuals with Down Syndrome.

    Neurobiology of disease·2001
    Same journal

    A pan-vertebrate signaling motif controls the molecular function of intracellular AQP12.

    The Journal of cell biology·2026
    Same journal

    Synergistic assembly, disassembly, and protection of complex forms of bundled F-actin.

    The Journal of cell biology·2026
    Same journal

    Recruitment and release of XPG during NER is controlled by pre- and post-incision factors and EXO1.

    The Journal of cell biology·2026
    Same journal

    Meiotic CENP-C supports centromere assembly and kinetochore recruitment in spermatogenesis.

    The Journal of cell biology·2026
    Same journal

    Phosphatidylserine and RhoB connect PI4P and PA metabolism to maintain plasma membrane identity.

    The Journal of cell biology·2026
    Same journal

    PIKfyve influences inter-organelle contacts with lysosomes to modulate the endoplasmic reticulum.

    The Journal of cell biology·2026
    See all related articles

    Synaptic complexes from rat brains were isolated. The postsynaptic density (PSD) integrity relies on polypeptides, as disrupting their bonds progressively degrades PSD structure.

    Area of Science:

    • Neuroscience
    • Cell Biology
    • Biochemistry

    Background:

    • Synaptic complexes are crucial for neuronal communication.
    • The postsynaptic density (PSD) is a key structure at the synapse.
    • Understanding PSD composition is vital for synaptic function research.

    Purpose of the Study:

    • To isolate synaptic complexes from rat brain.
    • To investigate the structural components of the postsynaptic density (PSD).
    • To determine the role of polypeptides in maintaining PSD integrity.

    Main Methods:

    • Isolation of synaptic complexes from rat brain tissue.
    • Treatment of isolated complexes with proteolytic enzymes, NaCl, EGTA, and urea at varying concentrations.
    • Microscopic and biochemical analysis to assess structural integrity and composition.

    Related Experiment Videos

    Main Results:

    • Synaptic complexes consist of pre- and postsynaptic membranes, a synaptic cleft, and a PSD.
    • Proteolytic enzymes degrade the PSD, while NaCl, EGTA, and mild urea treatments preserve it.
    • High urea concentrations partially dissociate the PSD, indicating polypeptide involvement.

    Conclusions:

    • Polypeptides are major components of the postsynaptic density (PSD).
    • The structural integrity of the PSD is dependent on polypeptide bonding (covalent or hydrophobic).
    • Disruption of these polypeptide bonds leads to progressive PSD structural loss.