High-performance liquid chromatography for ultraviolet-absorbing constituents in human urine

Several liquid-chromatographic systems involving anion-exchange resins and linear acetate gradient elution have been reported previously for the analysis of ultraviolet-absorbing constituents in human urine. We describe a high-performance liquid-chromatographic system on which a macroreticular anion-exchange resin, and stepwise elution are used. Separations were more rapid with ammonium chloride/acetonitrile stepwise elution than with acetate stepwise elution, and urine constituents could be detected at 225 nm with the former system. Analytical conditions were convenient and the method has proven to be useful for routine clinical analysis.