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Disappointment with triethers as markers for measuring triglyceride absorption in man
Gut
|November 1, 1972
Summary
Glycerol triethers were tested as markers for fat absorption but separated too quickly from the meal. This rapid separation in the stomach prevented accurate measurement of triglyceride absorption in the jejunum.
Area of Science:
- Gastroenterology
- Nutritional Science
- Biochemistry
Background:
- Accurate measurement of triglyceride absorption is crucial for understanding fat metabolism.
- Non-absorbable, oil-phase markers are needed to indirectly assess lipid digestion and absorption.
- Glycerol triethers were investigated as potential markers due to their chemical stability.
Purpose of the Study:
- To evaluate glycerol triethers as non-absorbable markers for quantifying triglyceride absorption in the human proximal jejunum.
- To assess the suitability of specific glycerol triethers (1-hexadecyl-2, 3-didodecyl glycerol, 1,2,3-trioleyl glycerol, 1,2,3-tridecyl glycerol) for this purpose.
Main Methods:
- Infusion of tritium-labeled glycerol triethers dissolved in linseed oil and egg yolk lipids into the stomach.
- Preparation of an aqueous emulsion of the lipid mixture for oral administration.
- Analysis of postprandial jejunal contents to determine the ratio of triether to triglyceride.
Main Results:
- Postprandial jejunal contents showed a lower proportion of glycerol triethers relative to triglycerides than in the initial test meals.
- Glycerol triethers demonstrated a faster rate of separation from the aqueous phase compared to triglycerides.
- This dissociation occurred within the stomach, prior to reaching the jejunum.
Conclusions:
- Glycerol triethers, under these conditions, are unsuitable as non-absorbable markers for indirect measurement of triglyceride absorption.
- The rapid phase separation of triethers in the stomach interferes with their use in this test system.
- Alternative markers or methodologies are required for accurate quantification of jejunal triglyceride absorption.
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