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Published on: October 8, 2012

Effects of furazolidone on the infection of Vibrio cholerae by bacteriophage phi149

S N Chatterjee, M Maiti

    Journal of Virology
    |June 1, 1973
    PubMed

    Insights

    Furazolidone significantly reduced phage phi149 yield in Vibrio cholerae, impacting DNA synthesis. The drug

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Bacteriology

    Background:

    • Bacteriophages are viruses that infect bacteria.
    • Furazolidone is an antimicrobial drug.
    • Understanding drug-host-phage interactions is crucial for antimicrobial resistance research.

    Purpose of the Study:

    • To investigate the effect of furazolidone on the replication of phage phi149 in Vibrio cholerae.
    • To determine the impact of furazolidone on bacterial and phage macromolecular synthesis.

    Main Methods:

    • Vibrio cholerae OGAWA 154 infected with phage phi149 were treated with varying concentrations of furazolidone.
    • Phage yield, burst size, latent period, and DNA, RNA, and protein synthesis were measured.
    • Phage adsorption to bacterial cells was analyzed.

    Main Results:

    • Furazolidone significantly reduced phage phi149 yield, despite the phage's in vitro resistance to the drug.
    • DNA synthesis inhibition was dose-dependent and more pronounced in infected cells.
    • Burst size decreased exponentially with increasing furazolidone concentration, while latent and eclipse periods remained unchanged.
    • Furazolidone did not affect phage receptors on bacterial cells.

    Conclusions:

    • Furazolidone interferes with phage replication in Vibrio cholerae, primarily by inhibiting DNA synthesis.
    • The timing of furazolidone exposure critically influences phage yield.
    • The drug's mechanism involves targeting host cell processes rather than direct antiviral action or receptor binding.

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