Tetrodotoxin desensitization in aggregates of embryonic chick heart cells

Spontaneous beating of heart-cell aggregates from 4-day chick embryos was initially blocked by 10(-5) g/ml tetrodotoxin (TTX). With continued exposure to the drug, the fraction of blocked aggregates decreased from about 80% at 15 min to about 25% at 2-3 h, at which time, beating aggregates had become desensitized to the toxin, showing no response to a fresh dose. Aggregates from 5-day hearts were more sensitive to TTX, but fewer became desensitized in its presence. Desensitization to TTX was not seen in 6- and 7-day aggregates. Inhibition of protein synthesis by cycloheximide did not affect beating or initial sensitivity to TTX of 4-day aggregates, but desensitization failed to occur. Before TTX, the mean value of maximal upstroke velocity (V(max)) of the action potentials in 4-day aggregates was 33 V/s. After desensitization V(max) was 12 V/s. Activity of desensitized aggregates in the presence of TTX was augmented by elevated calcium levels, and suppressed by presumed inhibitors of slow inward current (manganese, D600). Desensitization was reversible; upon removal of TTX 10(-5) g/ml, aggregates regained their responsiveness to a fresh dose of the drug with a 2-3 h time-course similar to that of desensitization. This was prevented by continued exposure to TTX at concentrations as low as 10(-8) g/ml. These data suggest that (a) desensitization involves a change in the mode of action-potential generating from one involving Na-specific, TTX-sensitive channels to one utilizing slower Mn-sensitive channels; (b) the process of desensitization occurs over a period of 2-3 h and is dependent upon the products of protein synthesis; and (c) desensitization is reversible after removal of TTX over a 2-3 h time-course similar to its onset.