Plastic casts of embryonic respiratory and cardiovascular system: a technique

This report describes a technique for producing plastic casts of the cardiovascular and respiratory systems of the chick embryo at Stage 36 or older. For casts of the cardiovascular system, polymerization compound is injected in the right ventricle filling the heart, venous, and arterial system. A two stage injection produced more detailed casts of the pulmonary vasculature. For respiratory system casts, the compound is injected in the trachea filling the bronchi and air sacs. Casts are prepared by tissue corrosion in 10% potassium hydroxide. This technique is an alternative to serial section reconstruction in the study of developing cardiovascular and respiratory system.