Glucose measurements. A 1977 CAP Survey analysis.

Area of Science:

• Clinical Chemistry
• Laboratory Medicine
• Analytical Chemistry

Background:

• Accurate glucose measurement is critical for diagnosing and managing diabetes.
• Interlaboratory quality control programs are essential for evaluating analytical methods.
• The 1977 College of American Pathologists (CAP) Comprehensive Chemistry Survey provides valuable data for method assessment.

Purpose of the Study:

• To analyze the performance of different glucose measurement methods in the 1977 CAP Comprehensive Chemistry Survey.
• To identify the most accurate and precise methods for glucose determination.
• To evaluate the suitability of various methods for both serum and urine glucose analysis.

Main Methods:

• Analysis of glucose measurement data from the 1977 CAP Comprehensive Chemistry Survey.
• Comparison of method-specific mean concentrations against all-method mean concentrations.
• Evaluation of accuracy and precision using interlaboratory comparison statistics.

Main Results:

• Hexokinase methods demonstrated the highest accuracy in glucose measurements.
• Glucose oxidase-oxygen rate methods exhibited the best interlaboratory precision.
• Neocuproine methods showed unacceptable positive biases; redox methods performed poorly and should be eliminated.
• Accuracy and precision were significantly lower for urine glucose measurements compared to serum.

Conclusions:

• Hexokinase and glucose oxidase-oxygen rate methods are recommended for accurate and precise glucose determination.
• Neocuproine and other redox methods are unsuitable for clinical glucose analysis due to poor performance.
• Method selection significantly impacts glucose measurement quality, especially between serum and urine samples.