Inhibition of murine bone marrow colonies growth by adenosine containing nucleotides

The effect of various compounds: NAD+, NADP+, Adenosine Diphospho-Ribose, Nicotinamide, Ribose 5 Phosphate, Adenosine, cAMP, cGMP, on the growth of granulocytic and erythroid mouse bone marrow colonies (CFUc and CFUE) was evaluated. The substances were either added directly to the semi-solid culture system, to obtain a continuous exposure during colony growth, or preincubated for various times with bone marrow cells before plating. The results indicate an inhibitory effect on the number of both colonies of several adenosine containing compounds. The effect is concentration dependent and irreversible. CFUE and CFUC show different sensitivity to the inhibition.