Immunocytological methods for the identification and localization of antigens

Immunocytologic techniques allow the study of antigenic cellular constituents by optical or electron microscopy, Antibodies labelled with tracers such as fluorochromes, enzymes, radioactive isotopes, or large electron-dense molecules form the basis of these techniques. The immunochemical procedures involved in the preparation of such conjugates are well defined. Satisfactory results, however, also depend upon the proper preparation and processing of the tissue or cell suspension being studied.