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Polyploidization and hemiploidization induced by PUVA in vivo
Dermatologica
|January 1, 1979
Summary
Photochemotherapy (PUVA) impacts skin cells by initially slowing proliferation, then normalizing it. It also causes various chromosomal abnormalities, including multinucleation and translocations, in patients with psoriasis.
Area of Science:
- Dermatology
- Cell Biology
- Genetics
Background:
- Psoriasis is a chronic inflammatory skin condition.
- PUVA therapy (psoralen plus ultraviolet A) is a common treatment for psoriasis.
- Understanding PUVA's in vivo effects on skin cells is crucial for assessing treatment safety.
Purpose of the Study:
- To investigate the in vivo effects of PUVA treatment on cutaneous cells from psoriatic patients.
- To analyze morphological and chromosomal modifications in skin cells following PUVA therapy.
Main Methods:
- Primary skin cell cultures were established from 15 psoriatic patients undergoing PUVA therapy.
- Biopsies were taken before treatment (control) and during therapy.
- Cells were analyzed for morphological and chromosomal changes.
Main Results:
- PUVA treatment initially inhibited cell proliferation, which later reverted to normal levels.
- Observed chromosomal abnormalities included multinucleation, macrocells, micronuclei, polyploidy, and haploidy.
- Specific chromosomal changes noted were a reduction to 13 chromosomes, end-associations, diplotene chromosomes, and translocations.
Conclusions:
- PUVA therapy induces significant morphological and chromosomal alterations in skin cells of psoriatic patients.
- These findings highlight the genotoxic potential of PUVA treatment.
- Further research is needed to fully understand the long-term implications of these cellular changes.