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Pyridine extraction method for determination of bacitracin in feeds: collaborative evaluation
Journal - Association of Official Analytical Chemists
|May 1, 1979
Summary
The pyridine extraction method for determining bacitracin in animal feed showed high variability and operational issues, primarily with pyridine evaporation. This suggests the method should be discontinued due to reliability concerns.
Area of Science:
- Analytical Chemistry
- Animal Nutrition
Background:
- Accurate quantification of feed additives like bacitracin is crucial for animal health and productivity.
- Existing analytical methods require validation for routine use in feed analysis.
Purpose of the Study:
- To evaluate the reliability and reproducibility of the pyridine extraction method for determining zinc and MD bacitracin in animal feed and premixes.
- To identify operational challenges associated with the pyridine extraction method.
Main Methods:
- Collaborative study involving 17 laboratories.
- Analysis of swine and broiler rations and premixes using the pyridine extraction method and neomycin-sensitized agar.
- Determination of bacitracin activity over two days with multiple dilutions.
Main Results:
- No significant differences in bacitracin activity were found between dilutions or days for individual samples.
- The type of bacitracin or feed did not significantly impact the results.
- High coefficients of variation (13% for premixes, 15-30% for feeds) indicated operational problems, mainly related to pyridine evaporation and potential bacitracin degradation during drying.
Conclusions:
- The pyridine extraction method, as described in 42.200 and 42.204, is not recommended for routine analysis due to significant operational difficulties and high variability.
- Discontinuation of this specific pyridine extraction method is advised for reliable bacitracin determination in animal feed.