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Related Experiment Videos

ECHOvirus 33 replication in human peripheral white blood cells.

J W Gnann, E C Hayes, J Z Smith

    Journal of Medical Virology
    |January 1, 1979
    PubMed
    Summary

    Echovirus 33 replicates in immune cells from healthy donors. However, immune cells from an agammaglobulinemia patient neutralized the virus, suggesting a role for antibodies in controlling echovirus infections.

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    Area of Science:

    • Virology
    • Immunology
    • Infectious Diseases

    Background:

    • Agammaglobulinemia is a primary immunodeficiency characterized by a lack of antibodies.
    • Echoviruses are a group of viruses that can cause various illnesses.
    • Understanding viral replication and host-pathogen interactions is crucial for developing effective treatments.

    Purpose of the Study:

    • To investigate the in vitro replication of Echovirus 33 in immune cells.
    • To compare the susceptibility of immune cells from normal donors and patients with agammaglobulinemia to Echovirus 33 infection.
    • To explore the role of antibodies in controlling Echovirus 33 infection.

    Main Methods:

    • Isolation of Echovirus 33 from cerebrospinal fluid of an agammaglobulinemia patient.

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  • In vitro replication studies using peripheral leukocyte suspensions from normal antibody-negative donors.
  • Culture of washed glass adherent mononuclear cells from normal antibody-negative donors.
  • Sequential dilution experiments to demonstrate viral replication.
  • In vitro studies using leukocyte suspensions from the infected agammaglobulinemia patient.

    • Main Results:

    • Echovirus 33 replicated in vitro in peripheral leukocyte suspensions from normal antibody-negative donors.
    • Washed glass adherent mononuclear cell cultures from normal antibody-negative donors supported Echovirus 33 replication.
    • Leukocyte suspensions from the infected agammaglobulinemia patient were capable of extinguishing detectable infectious virus in vitro.

    Conclusions:

    • Peripheral leukocytes and mononuclear cells from normal individuals can support Echovirus 33 replication in vitro.
    • Immune cells from patients with agammaglobulinemia demonstrate an ability to neutralize Echovirus 33 in vitro, suggesting a potential role for antibodies in viral clearance.