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Related Experiment Videos

Automated analysis of chlorthalidone.

M M Johnston, M Rosenberg, B Kamath

    Journal of Pharmaceutical Sciences
    |August 1, 1979
    PubMed
    Summary

    This study presents a new method for measuring chlorthalidone (a diuretic) in biological samples. The developed assay accurately quantifies chlorthalidone in urine and plasma, aiding in therapeutic drug monitoring.

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    Area of Science:

    • Pharmacology
    • Analytical Chemistry
    • Biochemistry

    Background:

    • Chlorthalidone is a widely prescribed diuretic.
    • Accurate quantification of chlorthalidone in biological fluids is essential for therapeutic drug monitoring and pharmacokinetic studies.
    • Existing methods may lack the sensitivity or throughput required for routine clinical analysis.

    Purpose of the Study:

    • To develop and validate a sensitive and efficient assay for the determination of chlorthalidone in human plasma and urine.
    • To establish a method suitable for analyzing chlorthalidone concentrations after therapeutic dosing.

    Main Methods:

    • Enzyme inhibition assay utilizing bovine erythrocyte carbonic anhydrase and p-nitrophenyl acetate as substrate.
    • Development of a fully automated, continuous flow system for urinary sample extraction and analysis.
    • Adaptation of a manual extraction procedure combined with a continuous flow system for plasma sample analysis.

    Main Results:

    • The automated urinary assay achieved a sensitivity of 0.5 µg/ml, enabling measurement after therapeutic doses.
    • The plasma assay, with a sensitivity of 25 ng/ml, could detect low therapeutic concentrations.
    • Both methods demonstrated high throughput, with over 100 urine samples analyzed per day by a single operator.

    Conclusions:

    • The developed enzymatic inhibition assay provides a sensitive and efficient method for chlorthalidone determination in both urine and plasma.
    • The automated system for urine analysis offers high throughput and practicality for clinical laboratories.
    • These assays are valuable tools for pharmacokinetic research and therapeutic drug monitoring of chlorthalidone.

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