Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

JC human papovavirus replication in human amnion cells.

K K Takemoto, P M Howley, T Miyamura

    Journal of Virology
    |April 1, 1979
    PubMed
    Summary

    JC virus, a human papovavirus, successfully replicated in primary human amnion cells. Further analysis confirmed its identity and DNA characteristics, matching the prototype strain.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Early use of allogeneic hematopoietic stem cell transplantation for infants with MLL gene-rearrangement-positive acute lymphoblastic leukemia.

    Leukemia·2014
    Same author

    Virological efficacy of combination therapy with corticosteroid and nucleoside analogue for severe acute exacerbation of chronic hepatitis B.

    Journal of viral hepatitis·2014
    Same author

    Sicca syndrome in patients infected with human immunodeficiency virus-1.

    Modern rheumatology·2014
    Same author

    Eternacept for the treatment of patients with rheumatoid arthritis and concurrent interstitial lung disease.

    Journal of clinical pharmacy and therapeutics·2010
    Same author

    Purification of intact microtubules from brain.

    The Journal of cell biology·2009
    Same author

    Prevalence of antibody to hepatitis E virus among wild sika deer, Cervus nippon, in Japan.

    Archives of virology·2007

    Area of Science:

    • Virology
    • Cell Biology
    • Molecular Biology

    Background:

    • Human papovaviruses, including JC virus, are known human pathogens.
    • Understanding viral replication mechanisms is crucial for developing antiviral strategies.

    Purpose of the Study:

    • To investigate the replication capability of JC virus in primary human amnion cells.
    • To characterize the replicated JC virus using serological and molecular methods.

    Main Methods:

    • Replication of JC virus in primary human amnion cells over eight passages.
    • Serological identification of the virus.
    • Restriction endonuclease analysis of viral DNA.

    Main Results:

    • JC virus demonstrated replication in primary human amnion cells.
    • Serological testing confirmed the viral identity as JC virus.
    • Restriction endonuclease analysis showed viral DNA fragments identical to the prototype strain.

    Conclusions:

    • Primary human amnion cells support JC virus replication.
    • The replicated JC virus maintains its characteristic DNA structure.
    • This study provides a foundation for further research into JC virus pathogenesis and cell tropism.

    Related Experiment Videos