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L-Glutamine formation by Flavobacterium rigense.

S Yamada, K Nabe, T Ujimaru

    Applied and Environmental Microbiology
    |June 1, 1979
    PubMed
    Summary
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    A novel mutant of Flavobacterium rigense, strain 703, was developed for enhanced L-glutamine production. This penicillin-resistant strain efficiently accumulates L-glutamine using ammonium fumarate as a key factor.

    Area of Science:

    • Microbiology
    • Biotechnology
    • Biochemistry

    Background:

    • The parent strain, Flavobacterium rigense FERM-P no. 3556, produces 0-2-hydroxypropylhomoserine.
    • Strain 703 is a penicillin-resistant mutant developed via ultraviolet treatment.
    • The mutant exhibits distinct physiological characteristics compared to typical L-glutamic acid producers.

    Purpose of the Study:

    • To characterize a novel penicillin-resistant mutant of Flavobacterium rigense.
    • To investigate the L-glutamine production capabilities of the mutant strain.
    • To identify optimal conditions for L-glutamine accumulation.

    Main Methods:

    • Generation of a penicillin-resistant mutant (strain 703) from Flavobacterium rigense using ultraviolet treatment.
    • Physiological characterization of the mutant, including growth requirements.

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  • Optimization studies for L-glutamine production, focusing on medium components and environmental factors.

    • Main Results:

    • Strain 703 was identified as a potent producer of L-glutamine.
    • The mutant's growth required L-tryptophan and thiamine, but not biotin.
    • L-glutamine formation was independent of L-tryptophan and thiamine, but significantly enhanced by ammonium fumarate.
    • Optimal accumulation reached 10 mg/ml L-glutamine under specific conditions (30°C, 48h, 3% diammonium fumarate).

    Conclusions:

    • Strain 703 represents a promising microbial resource for industrial L-glutamine production.
    • Ammonium fumarate is a critical factor for maximizing L-glutamine yield in this strain.
    • The unique physiological and nutritional requirements of strain 703 offer insights into bacterial metabolism and strain development.