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Operon coordination in different bacterial hosts.

P C Wu, P F Johnson, A Newton

    Journal of Bacteriology
    |August 1, 1970
    PubMed
    Summary
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    Gene expression in the lac operon differs between Escherichia coli and Salmonella typhimurium, particularly in transacetylase levels. Bacterial membrane structures do not significantly impact galactoside transport.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • The lac operon regulates lactose metabolism in bacteria.
    • Comparing gene expression in Escherichia coli and Salmonella typhimurium can reveal differences in protein synthesis and membrane structure.

    Purpose of the Study:

    • To compare the coordination of gene expression in the lac operon between E. coli and S. typhimurium.
    • To investigate potential differences in protein synthesis and membrane structure affecting lac operon function.

    Main Methods:

    • Introduction of wild-type or mutated F' lac pro(AB) episomes into pro(-) derivatives of E. coli and S. typhimurium.
    • Assays for beta-galactosidase, transacetylase, and galactoside transport activities.
    • Analysis of lac operon gene expression and membrane-associated transport systems.

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    Main Results:

    • Beta-galactosidase levels were slightly lower in S. typhimurium compared to E. coli.
    • Transacetylase levels were significantly higher in S. typhimurium across all tested lac markers.
    • Galactoside transport activities were comparable between the two bacterial strains, suggesting similar membrane structures.
    • Specific transport activity decreased rapidly in cultures past the mid-exponential growth phase for both bacteria.

    Conclusions:

    • While overall lac operon gene expression shows some differences, particularly in transacetylase, the membrane-associated galactoside transport system is functionally similar in E. coli and S. typhimurium.
    • Bacterial cell envelopes do not possess significant differences that would impede lac transport.
    • Culture age critically influences specific transport activity in both species, highlighting the importance of growth phase in experimental design.