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Related Experiment Videos

Rapid quantitative method for Salmonella detection in polluted waters.

C M Cheng, W C Boyle, J M Goepfert

    Applied Microbiology
    |April 1, 1971
    PubMed
    Summary
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    A new method rapidly enumerates salmonellae in polluted water samples within 48 hours. This technique enhances water quality monitoring by providing quick and reliable bacterial counts.

    Area of Science:

    • Environmental microbiology
    • Bacteriology
    • Water quality analysis

    Background:

    • Salmonellae contamination in water poses significant public health risks.
    • Accurate and rapid enumeration of salmonellae is crucial for effective water quality management.
    • Existing methods for salmonellae detection can be time-consuming and less efficient.

    Purpose of the Study:

    • To develop and validate a rapid and reliable procedure for enumerating salmonellae in polluted water.
    • To improve the efficiency of salmonellae detection in various water matrices.
    • To provide a timely method for assessing water contamination levels.

    Main Methods:

    • Modified selective enrichment using m-Tetrathionate Broth (22 ± 1 hr) at 41.5°C.
    • Plating on Brilliant Green Sulfa Agar (20 ± 1 hr) for bacterial isolation.

    Related Experiment Videos

  • Confirmation via flagellar (H) agglutination in a mannose-containing medium (6 ± 1 hr).
  • Concentration of large sample volumes using membrane filters.
  • Enumeration using dilution to extinction (most probable number) techniques.

    • Main Results:

    • Salmonellae confirmation achieved within a 48-hour timeframe.
    • The procedure demonstrated reliability across different water types, including wastewater and sludges.
    • Integration of membrane filtration allowed for efficient concentration of large sample volumes.

    Conclusions:

    • The developed procedure offers a rapid and reliable method for salmonellae enumeration in polluted waters.
    • This technique significantly reduces the time required for bacterial confirmation compared to traditional methods.
    • The method is suitable for routine monitoring of salmonellae in environmental water samples, wastewater, and sludges.