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Related Experiment Videos

R-factor transfer in selenite and tetrathionate broths.

P Gardner, A L Harding, R N Olans

    Applied Microbiology
    |April 1, 1971
    PubMed
    Summary
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    R-factor transfer between Escherichia coli and Salmonella typhimurium occurred in enrichment broths, but at a reduced frequency. This low frequency suggests minimal impact on interpreting R-factor studies despite potential for false resistance patterns.

    Area of Science:

    • Microbiology
    • Genetics
    • Antimicrobial Resistance

    Background:

    • R-factors, or resistance factors, are plasmids conferring antibiotic resistance.
    • Escherichia coli and Salmonella typhimurium are significant bacterial species.
    • Enrichment broths are commonly used for bacterial culture and diagnostics.

    Purpose of the Study:

    • To investigate the frequency of R-factor transfer between Escherichia coli and Salmonella typhimurium in specific enrichment broths.
    • To assess the potential impact of R-factor transfer in enrichment broths on diagnostic interpretations.

    Main Methods:

    • Overnight incubation of R(+)Escherichia coli with R(-)Salmonella typhimurium.
    • Use of selenite and tetrathionate with Brilliant Green (TBG) broths for incubation.

    Related Experiment Videos

  • Comparison of R-factor transfer frequency in TBG broths versus Trypticase soy broth.

    • Main Results:

    • R-factor transfer was demonstrated in 10 out of 12 experiments using TBG broths.
    • The frequency of R-factor transfer was markedly reduced in TBG broths compared to Trypticase soy broth.
    • The reduced frequency is attributed to adverse effects on bacterial viability or conjugation.

    Conclusions:

    • R-factor transfer in commonly used enrichment broths can occur, potentially leading to false antibiotic resistance patterns in Salmonella.
    • The low frequency of transfer in these broths makes significant impact on R-factor study interpretation unlikely.