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R-B enteric differential system for identification of Enterobacteriaceae
Applied Microbiology
|June 1, 1971
Summary
The R/B Enteric Differential System accurately identified an average of 89.6% of enteric bacteria. While useful for initial bacterial grouping, it is not a replacement for detailed identification methods.
Area of Science:
- Clinical microbiology
- Bacteriology
Background:
- Accurate identification of enteric bacteria is crucial for clinical diagnosis and epidemiological studies.
- Conventional identification methods can be time-consuming and require specialized expertise.
Purpose of the Study:
- To evaluate the performance of the R/B Enteric Differential System for identifying enteric bacteria.
- To determine the accuracy of the R/B system across different genera of Enterobacteriaceae.
Main Methods:
- The R/B Enteric Differential System was tested with 451 unknown bacterial cultures from the CDC stock culture collection.
- Identification accuracy was assessed without the use of serology.
Main Results:
- The R/B system achieved an average identification accuracy of 89.6% for enteric bacteria.
- Accuracy varied by genus, ranging from 47% for Klebsiella to 100% for Serratia and Providencia.
- Only three of eleven Enterobacteriaceae groups were identified with ≥95% accuracy.
Conclusions:
- The R/B Enteric Differential System is a reasonably successful screening tool for grouping bacteria.
- The system is not a substitute for more detailed conventional identification procedures.