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Tear-Derived Exosomal miR-15a as New Diagnostic Tool for Diabetic Retinopathy07:45

Tear-Derived Exosomal miR-15a as New Diagnostic Tool for Diabetic Retinopathy

This article describes a protocol for the collection and detection of miR-15a from tears as a new diagnostic tool for diabetic...
276
An Assay to Detect Protection of the Retinal Vasculature from Diabetes-Related Death in Mice04:36

An Assay to Detect Protection of the Retinal Vasculature from Diabetes-Related Death in Mice

A protection assay was developed to monitor the retinal vasculature's resilience to death from diabetes/diabetic retinopathy-related insults such as oxidative stress and cytokines.
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Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies12:28

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies

This article describes a well-established and reproducible lectin stain assay for the whole mount retinal preparations and the protocols required for the quantitative measurement of vascular parameters frequently altered in proliferative and non-proliferative...
4.2K
Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents09:28

Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents

Oxygen-induced retinopathy (OIR) can be used to model ischemic retinal diseases such as retinopathy of prematurity and proliferative diabetic retinopathy and to serve as a model for proof-of-concept studies in evaluating antiangiogenic drugs for neovascular diseases. OIR induces robust and reproducible neovascularization in the retina that can be...
9.3K
An Ex Vivo Tissue Culture Model for Fibrovascular Complications in Proliferative Diabetic Retinopathy08:10

An Ex Vivo Tissue Culture Model for Fibrovascular Complications in Proliferative Diabetic Retinopathy

Here, we present a protocol to study the pathophysiology of proliferative diabetic retinopathy by using patient-derived, surgically-excised, fibrovascular tissues for three-dimensional native tissue characterization and ex vivo culture. This ex vivo culture model is also amenable for testing or developing new...
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Optimized Ex-ovo Culturing of Chick Embryos to Advanced Stages of Development05:47

Optimized Ex-ovo Culturing of Chick Embryos to Advanced Stages of Development

Viewing and accessing the chicken embryo during development can be challenging. We have developed an ex ovo method that is simple, cost effective, and can easily be used in a classroom or research setting. This method provides access to the embryo into late stages of embryonic development (HH 40).
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