Effects of sample-diluent interactions on surface tension and pressures in continuous flow manifolds
Clinica Chimica Acta; International Journal of Clinical Chemistry
|November 15, 1979
Abstract:
When a plasma sample is aspirated into a continuous flow analyser the internal pressure of the manifold increases. The pressure change is the result of insufficient detergent present to bind all the protein in the sample, and is a potential source of inaccuracy and imprecision in the assay. It is recommended that Brij 35 be used at a concentration 10 times above the binding capacity of the albumin in the sample, to minimise changes in pressure between samples.
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