Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microbioassay of antimicrobial agents.

H J Simon, E J Yin

    Applied Microbiology
    |April 1, 1970
    PubMed
    Summary

    This study enhances an agar-diffusion microbioassay for antimicrobial agents, increasing sensitivity and applicability. The improved method accurately quantifies low antibiotic concentrations in small sample volumes, ideal for clinical pharmacology.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Uncoiling collagen: a multidimensional mass spectrometry study.

    The Analyst·2015
    Same author

    Laser profile reshaping in a Fabry-Perot thin film.

    Optics letters·2012
    Same author

    Temporal pulse reshaping with surface waves.

    Applied optics·2010
    Same author

    Observation of long range surface plasmon decay length by optical second harmonic generation.

    Applied optics·2010
    Same author

    Meig's syndrome; a case report and a review of recently published cases.

    American journal of obstetrics and gynecology·2010
    Same author

    Coherent backscattering of optical second-harmonic generation with long-range surface plasmons.

    Optics letters·2009

    Area of Science:

    • Microbiology
    • Pharmacology
    • Analytical Chemistry

    Background:

    • Agar-diffusion assays are standard for antimicrobial testing.
    • Existing methods may lack sensitivity for small sample volumes or broad antimicrobial ranges.

    Purpose of the Study:

    • To modify and enhance an agar-diffusion microbioassay for increased sensitivity and broader applicability.
    • To enable accurate quantification of antimicrobial agents in small sample volumes like serum and urine.

    Main Methods:

    • A modified agar-diffusion technique was developed.
    • Microbioassays were performed using 0.02 ml of test samples.
    • Standard curves were established in buffer and human serum.

    Main Results:

    • The microtechnique demonstrated up to a 20-fold increase in sensitivity compared to existing methods.
    • Assay error was less than +/-5% with high linearity.
    • The method was validated for numerous antimicrobials including penicillins, cephalosporins, and tetracyclines.

    Conclusions:

    • The enhanced microbioassay offers a sensitive, accurate, and adaptable method for quantifying antimicrobials.
    • This technique is particularly suitable for pediatric research and clinical pharmacology studies requiring small sample volumes.

    Related Experiment Videos