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Related Experiment Videos

Epidermal growth factor: high and low molecular weight forms.

J M Taylor, S Cohen, W M Mitchell

    Proceedings of the National Academy of Sciences of the United States of America
    |September 1, 1970
    PubMed
    Summary

    Epidermal growth factor (EGF) in mouse submaxillary glands exists as a high molecular weight complex. This complex can separate into EGF and an EGF-binding protein with arginyl-esterase activity.

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Cell Signaling

    Background:

    • Epidermal Growth Factor (EGF) is a potent mitogen involved in cell growth and differentiation.
    • The precise molecular form and interactions of EGF in biological tissues are crucial for understanding its function.
    • Submaxillary glands are known sources of biologically active molecules, including growth factors.

    Purpose of the Study:

    • To characterize the molecular form of Epidermal Growth Factor (EGF) in crude homogenates of the male mouse submaxillary gland.
    • To investigate the potential dissociation of EGF from associated proteins.
    • To identify the enzymatic activity of any associated binding proteins.

    Main Methods:

    • Analysis of crude submaxillary gland homogenates using molecular weight determination techniques.
    • Investigation of reversible dissociation of molecular complexes.
    • Assay for arginyl-esterase activity.

    Main Results:

    • Epidermal growth factor (EGF) activity was primarily detected in a high molecular weight complex (approximately 74,000 daltons).
    • This complex was shown to be reversibly dissociable into free EGF (approx. 6,400 daltons) and an EGF-binding protein (29,000 daltons).
    • The isolated EGF-binding protein exhibited significant arginyl-esterase activity.

    Conclusions:

    • EGF in mouse submaxillary gland homogenates exists predominantly as a complex with a binding protein.
    • This complex formation may regulate EGF activity or facilitate its storage.
    • The associated EGF-binding protein possesses arginyl-esterase activity, suggesting a potential functional link.

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