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Related Experiment Videos

Platelet counting with a laser nephelometer.

D J Giannitsis

    Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
    |August 1, 1979
    PubMed
    Summary
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    A novel laser nephelometric method offers accurate platelet counting. This technique demonstrates satisfactory sensitivity, reproducibility, and correlation with existing methods for routine laboratory use.

    Area of Science:

    • Clinical Biochemistry
    • Hematology
    • Analytical Chemistry

    Background:

    • Accurate platelet counting is crucial for diagnosing and monitoring various medical conditions.
    • Existing methods for platelet enumeration may have limitations in terms of speed, cost, or accuracy.
    • The development of new, reliable platelet counting techniques is essential for clinical laboratories.

    Purpose of the Study:

    • To describe a new method for platelet counting utilizing the laser nephelometric principle.
    • To evaluate the practical considerations for standardizing and correlating this new method.
    • To assess the applicability of the laser nephelometric method in routine biochemical laboratory settings.

    Main Methods:

    • Venous blood samples were collected in EDTA-NaCL solution.

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  • Blood was centrifuged at 100 g for 10 minutes.
  • A 1:80 dilution of the supernatant was prepared and measured using a nephelometer against a blank, with platelet counts determined via a standard curve.

    • Main Results:

    • The laser nephelometric method demonstrated satisfactory sensitivity and reproducibility.
    • The method showed good correlation with the established electronic coulter counting technique.
    • Experimental results support the practical application of this method in routine laboratory settings.

    Conclusions:

    • The described laser nephelometric method provides a viable and accurate approach for platelet counting.
    • The method is suitable for routine use in biochemical laboratories due to its satisfactory performance characteristics.
    • Further standardization and correlation studies confirm its reliability compared to existing methods.