Simultaneous assay for L-tryptophan, serotonin, 5-hydroxyindoleacetic acid, norepinephrine and dopamine in brain

A routine simultaneous assay for the title compounds is described, which uses a cation-exchange resin for separations, and standard fluorometric methods for analyses. Practicability of the ion-exchange chromatography is enhanced by means of a novel apparatus, and the procedure has the flexibility to permit extension to other endogenous compounds using published techniques.