Role of arachidonic acid derivatives in neutrophil aggregation: a hypothesis

Chemotactic substances stimulate neutrophils in suspension to aggregate. Arachidonic acid (but not several structurally related fatty acids) induces a similar neutrophil response. We now report that two blockers of arachidonic acid metabolism, indomethacin and 5,8,11,14-eicosatetraynoic acid, inhibit this arachidonic acid-mediated response. Moreover, both blockers also inhibit aggregation stimulated by a synthetic chemotactic tripeptide, formyl-methionyl-leucyl-phenylalanine, and their potency in doing so parallels their potency in inhibiting the response to arachidonic acid. These results suggest that metabolites of arachidonic acid may stimulate certain neutrophil functions and be involved in cellular responses to at least some chemotactic substances.