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Density gradient separation of marrow precursor cells restricted for antibody specificity.
Science (New York, N.Y.)
|July 9, 1971
Summary
Mouse bone marrow cells commit to specific antigens before interacting with thymus cells. These early immune cells are not yet restricted to producing specific antibody classes like immunoglobulin M or G.
Area of Science:
- Immunology
- Cell Biology
- Hematology
Background:
- Potentially immunocompetent cells in (C57BL/6 x DBA/2) F(1) mouse bone marrow show early commitment to specific antigenic determinants.
- This commitment occurs before interaction with thymus-derived cells and the initiation of adaptive immune responses.
Purpose of the Study:
- To investigate the differentiation stage of bone marrow cells committed to specific erythrocyte antigens.
- To determine if these early committed cells are restricted to particular antibody classes (immunoglobulin M or G).
Main Methods:
- Utilizing equilibrium centrifugation in discontinuous bovine serum albumin gradients.
- Separating bone marrow cell precursors based on their buoyant density.
Main Results:
- Precursors for antibody-producing cells against sheep erythrocytes migrated to denser regions.
- Precursors for antibody-producing cells against burro erythrocytes remained in lower density regions.
- These committed precursors were not yet restricted to immunoglobulin M or G antibody production.
Conclusions:
- Bone marrow cells commit to specific antigenic determinants early in differentiation.
- Density gradient centrifugation can distinguish between precursors committed to different erythrocyte antigens.
- Early committed immune cells are not yet restricted to specific antibody isotypes.